方丹君

发布时间:2020-07-01浏览次数:7033


方丹君

博士,教授博士生导师 

招生方向临床药学药学

联系方式Emaildjf@njmu.edu.cn

个人简介:

2010年毕业于美国凯斯西储大学化学系,获博士学位,期间从事单细胞电化学相关科研工作2011年作为高层次引进人才受聘于澳门威斯人0168临床药学系。科研方向包括:构建微纳电化学、电致化学发光等快速、高通量分析平台,对单细胞中生物分子进行特异性定性定量分析及成像,从而为相关疾病的早期诊断药物研发提供有效的生物学信息。教学主讲课程包括:基础化学临床药学英语医学英语MBBS留学生有机化学等。 

主持科研项目: 

1. 国家自然科学基金面上项目:单个活细胞膜中胆固醇分子跨膜转运的快速电致化学发光测量,20201月至202312月,65万元,在研

2. 国家自然科学基金面上项目高通量单细胞分析平台研究细胞表面胆固醇与脂分子的相互作用20161月至201912月,65万元,已结题

3. 国家自然科学基金青年项目快速单细胞胆固醇电分析平台的建立及其在斯达汀类药物开发中的应用,20121月至201412月,25万元已结题。 

4. 留学回国科技活动项目择优资助经费。

 

近年代表性论著

1. Yanyan Xu, Kang Liu, Rong Jin, Prof. Dechen Jiang*, Prof. Danjun Fang*, Dynamic Visualization of Free Radicals at Single Oxygen Bubbles using Chemiluminescence. Chemistry-An Asian Journal, 2021, 16, pp.4049-4052.

2. Dongni Han, Bertrand Goudeau, Dragan Manojlovic, Dechen Jiang, Danjun Fang,* and Neso Sojic*, Electrochemiluminescence Loss in Photobleaching. Angewandte Chemie International Edition, 2021, 60(14), 7686-7690.

3. Jingjing Zhang, Rong Jin, Yun Chen, Danjun Fang,* Dechen Jiang,* Enhanced electrochemiluminescence at single lithium iron phosphate nanoparticles for the local sensing of hydrogen peroxide efflux from single living cell under a low voltage. Sensors & Actuators, B: Chemical, 329(2021), 129208.

4. Dongni Han, Bertrand Goudeau,Dechen Jiang, Danjun Fang,* and Neso Sojic*. Electrochemiluminescence Microscopy of Cells: Essential Role of Surface Regeneration. Analytical Chemistry 2021, 93(3),1652−1657.

5. Kang Liu, Shuohan Huang, and Danjun Fang*. Electrochemical Measurement of Cholesterol Flip-Flop in Plasma Membrane at Single Cells. Analytical Chemistry 2020, 92(16), 10961−10965.

6. Priyanka Dutta, Dongni Han, Bertrand Goudeau, Dechen Jiang, Danjun Fang* , Neso Sojic*. Reactivity mapping of luminescence in space: Insights into heterogeneous electrochemiluminescence bioassays. Biosensors and Bioelectronics 165 (2020) 112372.

7. Jingjing Zhang, Yun Chen, Danjun Fang⁎, Electrochemiluminescence in Luminol-based calcium-selective nanoparticles for the determination of calcium ions. Journal of Electroanalytical Chemistry 878 (2020) 114671.

8. Zhang, Jingjing; Jin, Rong; Fang, Danjun*; Jiang, Dechen* , Voltage Modulated Electrochemiluminescence for Highly Sensitive Detection. Chemical Journal of Chinese Universities, 2020, 41(11): 2421-2425.

9. Kang Liu, Dongni Han, Shuohan Huang, Danjun Fang⁎. Enzyme-modified microelectrodes for electrochemical detection of sphingomyelin in the plasma membranes of single cells. Electrochemistry Communications 112 (2020) 106671.

10. Linyu Li, Kang Liu, and Danjun Fang*. Single Cell Electrochemiluminescence Analysis of Cholesterol in Plasma Membrane during Testosterone Treatment. Electroanalysis 2020, 32, 1–7.

11. Cheng Chen, Xin Dong, Kai-Heng Fang, Fang Yuan,Yao Hu, Min Xu, Yu Huang, Xixiang Zhang, Danjun Fang*, Yan Liu*. Develop a 3D neurological disease model of human cortical glutamatergic neurons using micropillar-based scaffolds. Acta Pharmaceutica Sinica B 2019;9(3):557–564.

12. Shuohan Huang, Kang Liu, Dechen Jiang, and Danjun Fang*. Codetermination of Sphingomyelin and Cholesterol in Cellular Plasma Membrane in Sphingomyelin-depletion-Induced Cholesterol Efflux. Analytical Chemistry 201991 (2), 1501–1506.

13. Meiling Zhang,  Linyu Li, Danjun Fang,*  Thomas J. Kelley,  and James D. Burgess*. Single Cell Titration-Type Assay for Plasma Membrane Cholesterol Chemical PotentialAnalytical Chemistry 2018, 90(9), 5903−5908.

 

发明专利:

1. 江德臣,方丹君,徐晶晶,电致化学发光成像方法快速分析单细胞内成分的方法,中国,201610237426.8。申请2016415日,授权2019312日。

2. 方丹君,江德臣,周俊宇,一种电致化学发光成像装置及其应用,中国,201410126893.4申请2014331日,授权2016831日。    

3. 方丹君,江德臣,姜晖,韩芳霏,一种基于鲁米诺和联吡啶钌的电位分辨电致化学发光检测方法及其应用,中国,201410104233.6 申请2014319日,授权2016217日。

4. 方丹君,江德臣,田春秀,马光中,韩芳霏,一种微电极阵列及其在检测单细胞表面活化胆固醇上的应用,中国,201310654110 .5申请2013125日,授权201585日。



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